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Infrared Imaging Systems

ACCU-SORT SYSTEMS, INC. Corporate Overview Accu-Sort is a world leader in designing, building, and installing engineered automatic identification solutions to help companies improve their material handling and distribution operations. We offer a...

AV6010 2D Bar Code Scanner Typical AV6010 bar code scanner and dimensioning solutions address these industries and applications: * Parcel and Postal Logistics *...

Power Technology, Inc. Power Technology, Inc. designs, manufactures, and distributes laser diode products for OEM analytical, biomedical, and industrial applications. Products include laser diode modules with wavelengths from 370 to 1600nm,...

Infrared Viewing Devices - IRVM The IRVM is a high performance CCD camera that allows for viewing and recording of radiation in the 400 to 1700nm spectral range. The IRVM features a...

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LI-COR Biosciences	In-Cell Western Assays: Phospho-p38 Detection in HeLa Cells in Response to ... ® Revised September, 2010. The most recent version of this protocol is posted at http://biosupport.licor.com I. Required Reagents ........................................ ........................................ ......... 2 II. Assay Example ........................................ ........................................ ............... 2 III. Experimental ...

LI-COR Biosciences	In-Cell Western Assays: Phospho-p53 Detection in COS Cells in Response to ... Phospho-p53 Detection in COS Cells in Response to Hydroxyurea ® Revised September, 2010. The most recent version of this protocol is posted at http://biosupport.licor.com. I. Required Reagents................................ ........................................ ..................2 II. Assay Example................................. ........................................ ...

LI-COR Biosciences	Nucleic Acid Stains (TO-PRO -3 and Syto 60) Used as Normalizing Agents in ... When performing the In-Cell Western assay, one uorescence channel is often used for normalization. This allows quantication data for the protein of interest to be corrected for well-to-well variation in cell num- ber, thereby increasing the overall accuracy of the assay. In many cases, a second protein is chosen for nor- malization. This protocol describes the use of a DNA stain in the 700 nm ...

LI-COR Biosciences	In-Cell Western Assays: Complete Apoptosis Assay Example Detailing the Seeding, ... Complete Apoptosis Assay Example Detailing the Seeding, Induction, and Detection of the HeLa Cellular Response to Anisomycin Treatment ® Revised September, 2010. The most recent version of this protocol is posted at http://biosupport.licor.com. I. Required Reagents................................ ........................................ ..................2 II. Apoptosis Assay ...

LI-COR Biosciences	In-Cell Western Assays: Complete Sample Protocol for Measuring IC50 of ... Complete Sample Protocol for Measuring IC50 of Inhibitor U0126 in NIH3T3 Responding to Acidic ® Revised September 2010. The most recent version of this protocol is posted at http://biosupport.licor.com I. Required Reagents................................ ........................................ ..................2 II. Sample Protocol................................ ...

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Infrared Imaging Systems Research Library

LI-COR Biosciences	In-Cell Western Assays: Complete Sample Protocol Detailing the Seeding, ... Complete Sample Protocol Detailing the Seeding, Stimulation, and Detection of the NIH3T3 Cellular Response to Acidic Fibroblast Growth Factor (aFGF-1) Developed for: Odyssey® Infrared Imaging System Odyssey Sa Infrared Imaging System ® Revised September, 2010. The most recent version of this protocol is posted at http://biosupport.licor.com. I. Required Reagents................................ ...

LI-COR Biosciences	Electrophoretic Mobility Shift Assay (EMSA) Using IRDye® Oligonucleotides Gel shift assays or electrophoretic mobility shift assays (EMSA) provide a simple method to study DNA- protein interactions. This assay is based on the principle that a DNA-protein complex will have different mobility during electrophoresis than non-bound DNA. These shifts can be visualized on a native acrylamide gel using labeled DNA to form the DNA-protein binding complex. To date, protocols ...

LI-COR Biosciences	Fluorophore-Linked Immunosorbent Assay (FLISA) Recommendations Published January, 2006. The most recent version of this Technical Note is posted at http://biosupport.licor.com/support. Notice: LI-COR provides only limited tech- nical support for FLISA applications. Each FLISA assay will require individual optimi- zation and performance may vary. The following should be used only as a guideline for adapting a Fluorophore-Linked Immunosorbent Assay (FLISA) ...

LI-COR Biosciences	Good Westerns Gone Bad: Troubleshooting to Make Your NIR Westerns Great Please refer to your manual to confirm that this protocol is appropriate for the applications compatible with your model of Odyssey Imager. Published December 2008. Revised March 2011. The most recent version of this document is posted at http://biosupport.licor.com Pages I. Introduction to Western Blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 II. Factors That ...

LI-COR Biosciences	In-Cell Western Assays: FAQs for Suspension Cells ® Revised September, 2010. The most recent version of this protocol is posted at http://biosupport.licor.com. Page 1 — FAQs For Suspension Cells For In-Cell Western™ Protocols 1.1 How do you culture suspension cells? For instructions on culturing suspension cells, look up your cell line at http://www.atcc.org/ and follow the guidelines. 1.2 How do you make non-adherent cells (suspension cells) ...

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